qiagen 204143實(shí)時(shí)定量PCR試劑盒
qiagen 204143實(shí)時(shí)定量PCR試劑盒
QuantiTect SYBR Green PCR Kit使用SYBR Green I通過(guò)兩步法real-time RT-PCR對(duì)cDNA目標(biāo)序列進(jìn)行高特異性的定量檢測(cè)。熱啟動(dòng)和*的實(shí)時(shí)定量PCR緩沖液確保了對(duì)cDNA目標(biāo)序列高特異性和靈敏度的real-time定量檢測(cè)。dNTP混合液含有dUTP,可選擇性進(jìn)行UNG處理。為便于使用,預(yù)混液可儲(chǔ)存在2–8°C。
qiagen 204143實(shí)時(shí)定量PCR試劑盒
特異性定量檢測(cè)。
在Rotor-Gene 3000上使用針對(duì)人BAX的QuantiTect Primer Assay和的試劑盒分析稀釋的人白細(xì)胞cDNA。熔解曲線分析中出現(xiàn)單峰,NTC反應(yīng)呈平直曲線,說(shuō)明使用QuantiTect Kit和Assay可實(shí)現(xiàn)高特異性定量。NTC:無(wú)模板對(duì)照。Norm. Fluoro.:標(biāo)準(zhǔn)化的熒光。
at No./ID: 204141 QuantiTect SYBR Green PCR Kit (40) Trial kit for 40 x 50 µl reactions: 1 ml 2x QuantiTect SYBR Green PCR Master Mix, 2 ml RNase-Free Water |
Cat No./ID: 204143 QuantiTect SYBR Green PCR Kit (200) For 200x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water |
Cat No./ID: 204145 QuantiTect SYBR Green PCR Kit (1000) For 1000 x 50 µl reactions: 25 ml 2x QuantiTect SYBR Green PCR Master Mix, 20 ml RNase-Free Water |
Cat No./ID: 204163 QuantiTect SYBR Green PCR +UNG Kit (200) For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green PCR Master Mix, 100 µl UNG Solution, 2 x 2 ml |